Coding

Part:BBa_K3121007:Design

Designed by: Anish Ganju   Group: iGEM19_IISER_Bhopal   (2019-10-21)


Trigger Factor Frigidicola


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 10
    Illegal XbaI site found at 1372
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10
    Illegal BglII site found at 358
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 10
    Illegal XbaI site found at 1372
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 10
    Illegal XbaI site found at 1372
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1068


Design Notes

Codon Optimized for Escherichia coli, but could not be cloned.


Source

This was synthesized after its sequence was taken from literature sources. This part was codon optimized before synthesis

References